Locomotion of human lymphoid cells. I. Effect of culture and con A on T and non-T lymphocytes. |
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Authors: | G J O'Neill D M Parrott |
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Affiliation: | Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021 U.S.A. |
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Abstract: | Human peripheral lymphocytes were separated from whole blood on a Ficoll-Hypaque gradient. They were then depleted of monocytes, separated into T and non-T fractions, and assayed for locomotor responses toward casein and endotoxin-activated serum in Boyden chambers. Non-T cells showed higher random motility than did T cells. Culture prior to assay was necessary in order to demonstrate locomotor activity of T cells, but this requirement, although desirable, was not essential for non-T lymphocytes. It was not necessary for Con A to be present in the culture medium or for either T or non-T lymphocytes to be in blast form to show locomotion. |
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Keywords: | To whom all correspondence should be addressed. |
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