Histopathological indices and inflammatory response in the digestive gland of the mussel Mytilus galloprovincialis as biomarker of immunotoxicity to silver nanoparticles |
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| Authors: | Younes Bouallegui Ridha Ben Younes Houda Bellamine Ridha Oueslati |
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| Institution: | 1. Research Unit of Immuno-Microbiology Environmental and Cancerogensis, Sciences Faculty of Bizerte, University of Carthage, Tunisia;2. Pathological Anatomy Department, Regional Hospital of Menzel Bourguiba, Bizerte, Tunisia |
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| Abstract: | Background: Histopathological assessments approaches in bivalves have become an important tool in environmental toxicology. This study seeks to develop a quantitative histopathological index (Ih) and inflammation score as biomarkers in the aim to assess the health status of nanoparticles exposed mussels.Methods: Digestive gland hematoxylin and eosin (H&;E) stained sections from Mytilus galloprovincialis were assessed after in vivo exposure (for 3, 6 and 12?h) to silver nanoparticles (Ag-NPs?Results: Silver nanoparticles clearly induced histopathological alterations in digestive gland (maximum inflammation 2.75 with AgNP?p?Ih with AgNP?p?Ih were recorded after uptake routes were blockade: AgNP?p?Conclusions: Histopathological assessments showed to be promising tool in nanotoxicity which seems to depend on nanoparticles size, exposure time and interestingly to uptake routes. It was not clear: is it the length of exposure or the size of particles is more impactful. |
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| Keywords: | Inflammatory response histopathological alteration digestive gland nanotoxicity Mytilus galloprovincialis |
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