Simultaneous inhibition of contractile ring and central spindle formation in mammalian cells treated with cytochalasin B |
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Authors: | Daniela Cimini Daniela Fioravanti Caterina Tanzarella Francesca Degrassi |
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Institution: | (1) Dipartimento Genetica e Biologia Molecolare, Università“La Sapienza”, Via degli Apuli 4, I-00185, Roma, Italy, IT;(2) Dipartimento Biologia, Università“Roma Tre”, Rome, Italy, IT |
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Abstract: | In this work we have used the inhibitor of F-actin polymerisation cytochalasin B (Cyt B) to test the hypothesis that the contractile
ring and the central spindle are mutually interdependent structures in mammalian mitotic cells. Double fluorescence staining
of α-tubulin and F-actin was employed to analyse anaphase and telophase figures from asynchronously growing cultures and prometaphase-synchronised
cells. Testing for the presence of the central spindle and contractile ring in human primary fibroblasts, human hepatoma cells
and Chinese hamster cells after Cyt B treatment showed that both structures were simultaneously absent in over 60% of treated
anaphases and 80% of telophases. Experiments on resumption of cytokinesis in cleavage-arrested cells further showed that Cyt
B-treated human fibroblasts proceeded to cleavage within minutes after removal of the drug from the medium, concomitant with
the re-formation of both cellular structures in cleaving cells. These data suggest that the presence of a correctly assembled
contractile ring is essential for the formation and persistence of the central spindle during ana-telophase and provide further
support for the idea of a strong co-operative interaction between these two structures during cytokinesis.
Received: 7 August 1998 / Accepted: 13 September 1998 |
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