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ESR-spectroscopy of cytochrome P-450 LM2 in the soluble and membrane-bound state |
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| Authors: | V Iu Uvarov R M Davydov V P Timofeev |
| Abstract: | Influence of microenvironment on the structure of spin-labeled cytochrome P-450 LM2 was studied. The distance between the spin-label which is covalently bound to cysteine-152 and heme ferrum in soluble protein is 17 A and 23 A in the membrane-bound one. The spin-label was exposed in water solution in both cases. It was shown that solubilized cytochrome P-450 LM2 in water solution forms the aggregates consisting of 4-6 monomers. |
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