A simplified method for estimating ammonium oxidising bacteria |
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| Authors: | S. U. Sarathchandra |
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| Affiliation: | (1) Soil and Field Research Organisation, Ruakura Agricultural Research Centre, Hamilton, New Zealand |
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| Abstract: | Summary Most probable number of ammonium oxidising bacteria in soil has been estimated without the need to carry out chemical tests to determine the production of nitrite or nitrate in dilution tubes. The medium used contained phenol red which changes colour from pink to yellow as the oxidation of ammonium to nitrate decreases the pH. This makes it possible to determine growth visually. An incubation period of 8 weeks at 25°C was sufficient to obtain maximum estimates of ammonium oxidising bacteria in the soils tested. |
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| Keywords: | Ammonium oxidising bacteria Most probable number Nitrification Nitrosomonas nubers Soil |
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