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外源阿魏酸对离体培养棉纤维生长发育的影响
引用本文:吕萌,倪志勇,王娟,胡文冉,范玲. 外源阿魏酸对离体培养棉纤维生长发育的影响[J]. 西北植物学报, 2011, 31(4): 829-833
作者姓名:吕萌  倪志勇  王娟  胡文冉  范玲
作者单位:新疆农业科学院,核技术生物技术研究所,乌鲁木齐,830091
基金项目:农业部转基因重大专项课题,国家自然科学基金项目,国家"863"项目
摘    要:通过建立新疆陆地棉品种'新陆早36号'的体外胚珠培养棉纤维体系,利用图像数字化处理技术和电镜切片观察,探索体外培养条件下不同浓度外源阿魏酸(FA)对棉纤维生长发育的影响.结果表明:(1)随着培养时间的延长,培养棉胚珠的体积、棉纤维长度、纤维团生长面积和纤维细胞壁厚度均显著增加.(2)FA的影响在培养15 d后达到显著水...

关 键 词:阿魏酸(FA)  离体培养棉纤维  图像数字化处理  电镜切片

Development of the Cotton Fiber in vitro Mediated by Exogenous Ferelic Acid
L Meng,NI Zhi-yong,WANG Juan,HU Wen-ran,FAN Ling. Development of the Cotton Fiber in vitro Mediated by Exogenous Ferelic Acid[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(4): 829-833
Authors:L Meng  NI Zhi-yong  WANG Juan  HU Wen-ran  FAN Ling
Affiliation:L(U) Meng,NI Zhi-yong,WANG Juan,HU Wen-ran,FAN Ling
Abstract:By founded an ovlue culture system in vitro with ’Xinluzao 36’ which planted in Xinjiang,we studied the effects of exogenous ferelic acid(FA) in different concentrations on cotton fiber development using the technology of image digitization and electron microscopic observation.The results indicated that all the morphology index including ovular volume,fiber length,growing area and cell wall thickness increased.The effects of FA waxed after 15 day culture.FA concentration at 25 μmol/L and 50 μmol/L can both increase the fiber growth and cell wall thickness,but the incremental extents were seperated.Ovlues were observed and analyzed after 25 day culture,the data revealed that FA concentration at 25 μmol/L can promote the fiber elongation to increase 14.2%(P<0.01) and the growth area to increase 18%(P<0.01).While conspicuous cell wall thickening was observed when FA concentration raised up to 50 μmol/L,compared with the control,the thickness of the fiber cell wall raised 61.7%.
Keywords:ferulic acid(FA)  cotton fiber in vitro  image digitization  electron microscopic section  
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