Preparative separation of human B and T lymphocytes by free flow electrophoresis |
| |
Authors: | T G Baier G Weber K Hartmann U Heinrich D Sch?nberg |
| |
Affiliation: | Department of Pediatrics, University of Heidelberg, Federal Republic of Germany. |
| |
Abstract: | An electrophoretic method for the quantitative separation of human B and T lymphocytes in a carrier-free system is presented. The method is based on the fact that B and T lymphocytes show marked overlap in their size and density characteristics, but differ sufficiently in surface charge to be separable by electrophoresis. The technique is performed in phosphate-buffered saline and appears to be especially suitable for the enrichment of nonstimulated, functionally intact lymphocytes which can be directly used for further immunological or biochemical studies. |
| |
Keywords: | |
|
|