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| 中药红曲基原真菌的酯酶同工酶分析* | |
| 引用本文： | 邢旺兴, 宓鹤鸣, 陈士景, 程荣珍,. 中药红曲基原真菌的酯酶同工酶分析*[J]. 微生物学通报, 2000, 27(6): 437-440 |
| 作者姓名： | 邢旺兴 宓鹤鸣 陈士景 程荣珍 |
| 作者单位： | 1. 第二军医大学药学院药物分析教研室,上海,200433 2. 解放军117医院药剂科,杭州,310013 3. 杭州尖峰德康药业有限公司,杭州,310023 |
| 基金项目： | 解放军95'重点课题基金(No.96-Z-059) |
| 摘 要： | 探讨中药红曲基原真菌的同工酶分析方法。用凝胶电泳法分析７种红曲霉菌的胞内和胞外酯酶同工酶，模糊聚类法分析供试菌间的亲缘关系。红曲霉的酯酶同工酶胞内酶明显多于胞外酶，种间存在明显差异。同工酶分析可以作为红曲霉菌传统分类的重要辅助手段。 |
| 关 键 词： | 红曲 红曲霉菌 同工酶 分类 |
| 文章编号： | 0253-2654(2000)06-0437-04 |
| 修稿时间： | 1999-07-05 |
ANALYSIS OF ESTERASE ISOENZYME OF THE ORIGIN FUNGI OF THE CHINESE TRADITIONAL MEDICINE HONGQU |
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| XING Wang-Xing,MI He-Ming,CHEN Shi-Jing,CHENG Rong-Zhen. ANALYSIS OF ESTERASE ISOENZYME OF THE ORIGIN FUNGI OF THE CHINESE TRADITIONAL MEDICINE HONGQU[J]. Microbiology China, 2000, 27(6): 437-440 | |
| Authors: | XING Wang-Xing MI He-Ming CHEN Shi-Jing CHENG Rong-Zhen |
| Abstract: | Establish a analysis method of isoenzyme to classification of origin fungi of the Chinese traditional medicine, Hongqu. Established a gel electrophoresis method to analysis of esterase isoenzyme of 7 ordinary species Monascus and analyses the phylogenetic relationship by clustering analysis. The number of bands of intracelluar isoenzyme of Monascus are more than that of extracellular isoenzyme. There exist obviously differences between species. The analysis of isocnzyme is a important secondary technique being able to using to classify and identify of Monascus fungi. |
| Keywords: | Hongqu Monascus Isoenzyme Classification |
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