Biochemical Analysis of a Mutant Tetrahymena Lacking Outer Dynein Arms |
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Authors: | SUSAN A LUDMANN ANITA SCHWANDT XUEJUN KONC CONNIE S BRICKER DAVID G PENNOCK |
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Institution: | Department of Zoology, Miami University, Oxford, Ohio 45056;Department of Botany, Miami University, Oxford, Ohio 45056 |
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Abstract: | ABSTRACT. Tetrahymena thermophila mutants homozygous for the oad mutation become nonmotile when grown at the restrictive temperature, and axonemes isolated from nonmotile mutants lack approximately 90% of their outer dynein arms. Electrophoretic analyses of axonemes isolated from nonmotile mutants ( oad axonemes) indicate they contain significantly fewer of the 22 S dynein heavy chains that axonemes isolated from wild-type cells (wild-type axonemes) contain. The 22 S dynein heavy chains that remain in axonemes isolated from nonmotile, oad mutants are assembled into 22 S dynein particles that exhibit wild-type levels of ATPase activity. Two-dimensional gel electrophoresis of oad axonemes show that they are deficient in no proteins other than those proteins thought to be components of 22 S dynein. This report is the first formal proof that outer dynein arms in Tetrahymena cilia are composed of 22 S dynein. |
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Keywords: | Axoneme cilia ciliate temperature-sensitive oad mutation 22 S dynein |
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