| 环介导等温扩增核酸技术及其在食品安全检测领域的应用 |
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| 引用本文： | 杨小鹃,吴清平,张菊梅,张淑红,徐晓可.环介导等温扩增核酸技术及其在食品安全检测领域的应用[J].微生物学通报,2010,37(8):1227-1233. |
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| 作者姓名： | 杨小鹃 吴清平 张菊梅 张淑红 徐晓可 |
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| 作者单位： | 广东省微生物研究所,广东,广州,510070;广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东,广州,510070 |
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| 基金项目： | 广东省农业领域科技计划项目重点专项(No. 2009A020101004); 广东省科学院青年科学研究基金(No. qnjj20099) |
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| 摘 要： | 环介导等温扩增(Loop-mediated isothermal amplification,简称LAMP)是利用能识别靶序列上6个位点的4个特殊设计的引物和一种具有链置换活性的DNA聚合酶,在恒温条件下,特异、高效、快速地扩增核酸的新技术。该技术在1h内扩增效率可达到109-1010个数量级,扩增产物是一系列反向重复的靶序列构成的茎环结构和多环花椰菜样结构的DNA片段混合物,电泳后在凝胶上显现出由不同大小的区带组成的阶梯式图谱。近年来LAMP技术以其特异性强、等温灵敏、操作简单、产物易检测等优点已经应用于食品安全检测领域的多个方面。
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| 关 键 词： | 环介导等温扩增 LAMP 食品安全 检测 |
Loop-mediated Isothermal Amplification Method for Detection of Nucleic Acids and Its Application in Food Safety Inspection |
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| YANG Xiao-Juan,WU Qing-Ping,ZHANG Ju-Mei,ZHANG Shu-Hong and XU Xiao-Ke.Loop-mediated Isothermal Amplification Method for Detection of Nucleic Acids and Its Application in Food Safety Inspection[J].Microbiology,2010,37(8):1227-1233. |
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| Authors: | YANG Xiao-Juan WU Qing-Ping ZHANG Ju-Mei ZHANG Shu-Hong and XU Xiao-Ke |
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| Institution: | 1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China;1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China;1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China;1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China;1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China |
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| Abstract: | Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplified DNA with high specificity, efficiency and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. The cycling reaction continues with accumulation of 109-1010 copies of target in less than an hour. The final products are stem-loop DNA with several inverted repeats of the target and cauliflower-like structures with multiple loops. A positive reaction would be shown as a ladder-like pattern in a gel electrophoresis analysis. Because of its simplicity, high sensitivity and specificity, the LAMP method has been widely applied to the field of food safety inspection. |
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| Keywords: | Loop-mediated isothermal amplification LAMP Food safety Inspection |
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