| 酿酒酵母Saccharomyces cerevisiae重组菌株木糖醇发酵的初步研究 |
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| 引用本文： | 鲍晓明,郑华军,秦玉静,汪天虹,高东,李在禄.酿酒酵母Saccharomyces cerevisiae重组菌株木糖醇发酵的初步研究[J].工业微生物,2000,30(2):13-18. |
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| 作者姓名： | 鲍晓明 郑华军 秦玉静 汪天虹 高东 李在禄 |
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| 作者单位： | 1. 山东大学微生物技术国家重点实验室,济南,250100
2. 济南天恒生物工程中心,济南,250100 |
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| 基金项目： | 国家自然科学基金，39770012， |
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| 摘 要： | 木糖还原酶催化木糖为木糖醇的反应，是木糖代谢的第一步。将木糖还原酶的原因ＸＹＬ１引入酿酒酵母中，构建得到儿表达ＸＹＬ１基因的重组酿酒酵母菌株ＨＹＥＸ２，该重组菌株的木糖还原酶比活力为７．４７Ｕ／ｍｇ。研究表明，该菌株获得转化木糖产生木糖醇的能力，当辅助碳源葡萄糖的浓度为２％，并在发酵３０ｈ左右添加木糖，木糖醇的转化率可达到０．９７ｇ／ｇ。
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| 关 键 词： | 木糖还原酶基因 木糖醇 酿酒酵母 甜味剂 重组菌 |
Primary study on xylitol production by recombinant Saccharomyces cerevisiae strain |
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| BAO Xiao-ming,ZHENG Hua-jun,QIN Yu-jing,WANG Tian-hong,GAO Dong,LI Zai-lu.Primary study on xylitol production by recombinant Saccharomyces cerevisiae strain[J].Industrial Microbiology,2000,30(2):13-18. |
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| Authors: | BAO Xiao-ming ZHENG Hua-jun QIN Yu-jing WANG Tian-hong GAO Dong LI Zai-lu |
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| Abstract: | The first enzymatic step in xylose metabolism was catalyzed by xylose reductase (XR), which converted xylose to xylitol. The recombinant xylitol producing S. cerevisiae strain HYEX2 was constructed by transforming the XYL1 gene, which encoding XR. The specific activity of recombinant enzyme was 7.470U/mg protein. The result showed that 2% glucose was supplied as co substrate and xylose was added in 30 hours, the HYEX2 strain gave 0.94g/g xylitol yield. |
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| Keywords: | xylose reductase xylitol recombinant strain Saccharomyces cerevisiae |
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