Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1 |
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Authors: | L Sim O P Ward Z-Y Li |
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Institution: | (1) Microbial Biotechnology Laboratory, Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA |
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Abstract: | Pseudomonas aeruginosa UW-1 produced 17–24 g L−1 rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days. In time course studies of growth and
rhamnolipid production in a salts medium containing 6% canola oil, total bacterial count reached 2.6 × 1010 CFU ml−1 after 48 h and a maximum rhamnolipid yield of 24.3 g L−1 was obtained after 9 days. Rhamnolipid components were purified and separated by chloroform-methanol extraction and TLC
chromatography. The major rhamnolipid components were characterised as L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate and
L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate by nuclear magnetic resonance and mass spectrometry. The components
were separated preparatively by silica gel column chromatography. The recovered monorhamnosyl fraction contained no dirhamnosyl
moiety while the recovered dirhamnosyl fraction contained 5% of the monorhamnosyl moiety when analyzed by HPLC. The ratio
of mono- to dirhamnosyl components produced by P. aeruginosa UW-1 was determined by HPLC to be 4 : 1 by weight. Purified mono- and dirhamnosyl components had the same CMC value of
40 μg ml−1 and decreased the surface tension of water to 27.7 and 30.4 dynes cm−1, respectively.
Received 04 April 1997/ Accepted in revised form 15 July 1997 |
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Keywords: | : Pseudomonas aeruginosa rhamnolipid biosurfactant fermentation purification |
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