The streaming of the submandibular gland II: parenchyma and stroma advance at the same velocity

Abstract Thirty young male rats aged 7 weeks, weighing 200 g, were injected with 18.5 kBq g⁻¹ (0.5 μCi g⁻¹) body weight tritiated thymidine ³H]TdR (specific activity 185 GBq). The rats were then killed in groups of five, at the following times: 1 hour, and 14, 30, 60, 90, and 120 days. Autoradiograms of sections through the submandibular gland were prepared, and the location of labelled cells in tubular and acinar cross sections was recorded. The nuclear content of each cross section was defined as its 'class'. In this numbering system, narrow tubuli, e.g. intercalated ducts are of low class, and wider tubuli, e.g. striated ducts, of high class. One hour after labelling most labelled tubular cells were found in low class cross sections, i.e. intercalated ducts and narrow granular ducts. Striated ducts were not labelled. From then onward labelled cells entered wider tubuli, e.g. striated ducts. The advancing labelled epithelium was accompanied by labelled stroma. Both cell types traversed 0.089 classes per day. In acini, labelled cells advanced in the oposite direction, starting from acinar cross sections of high class and ending in class-1 cross sections.