Resolution of N-(2-ethyl-6-methylphenyl) alanine catalyzed by Lipase B from Candida antarctica |
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Authors: | Liangyu Zheng Suoqin Zhang Gui Gao Lifang Zhao Fei Jia Shugui Cao |
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Affiliation: | a Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, People's Republic of Chinab College of Chemistry, Jilin University, Changchun, People's Republic of China |
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Abstract: | A biotransformation process has been developed for the production of (S)-N-(2-ethyl-6-methylphenyl) alanine by enantioselective hydrolysis of racemic methyl ester in the presence of Candida antarctica lipase B (CAL-B). However, the enantioselectivity of CAL-B towards the resolution is not high enough to obtain enantiomerically pure product. In order to improve the enantioselectivity of the enzyme, the effects of surfactants on CAL-B-catalyzed hydrolysis were tested. The results suggest that surfactants can influence the microenvironment of the enzyme, and the addition of Tween-80, in particular, to the reaction medium markedly enhanced the selectivity of CAL-B towards the substrate used, with the enantiomeric ratio (E-value) increasing from 11.3 to 60.1. |
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Keywords: | Candida antarctica lipase B resolution (S)-N-(2-ethyl-6-methylphenyl) alanine surfactants |
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