Comparative aspects of sperm membrane fatty acid composition in silver (Vulpes vulpes) and blue (Alopex lagopus) foxes, and their relationship to cell cryopreservation |
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Authors: | Miller R R Cornett C L Waterhouse K E Farstad W |
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Institution: | Hillsdale College, Biology Department, 33 E. College, Hillsdale, MI 49242, USA. bob.miller@hillsdale.edu |
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Abstract: | Cryogenic protocols have been developed for the storage of farmed silver fox (Vulpes vulpes) spermatozoa. However, these same protocols and modifications of these protocols have failed to satisfactorily preserve spermatozoa collected from farmed blue foxes (Alopex lagopus). Because cryogenic success has been linked to membrane composition, the plasma membrane lipid composition of farmed blue fox and silver fox spermatozoa was studied. Silver fox spermatozoal membranes have significantly higher levels of docosapentaenoic acid (DPA; 22:5, n-6) compared to blue fox spermatozoa, and blue fox spermatozoal membranes have significantly higher levels of stearic acid (18:0). Silver fox spermatozoal membranes not only have a higher ratio of unsaturated/saturated membrane fatty acids, but also higher levels of membrane desmosterol and cholesterol. |
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Keywords: | Silver fox Vulpes vulpes Blue fox Arctic fox Alopex lagopus Spermatozoa Membranes Lipids |
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