Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli |
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Authors: | B. Gitter R. Diefenbach H. Keweloh D. Riesenberg |
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Affiliation: | (1) Hans-Knöll-Institut für Naturstoff-Forschung e.V., Beutenbergstrae 11, D-07745 Jena, Germany;(2) Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Corrensstrae 3, D-48149 Münster, Germany |
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Abstract: | In contrast to stringent (relA+) cells of Escherichia coli, relaxed (relA) cells excreted recombinant proteins (-lactamase, interferon 1) into the culture medium during amino acid limitation. Comparative analyses of overall fatty acid composition in relA+ cells and relA cells were performed and revealed that, in wild-type cells, drastic alterations occurred during the stringent response. The portion of saturated fatty acids (C16:0) and the fractions of cyclopropane fatty acids (C17cyc and C19cyc) increased whereas the portions of unsaturated fatty acids (C16:1 and C18:1) decreased. In cells of the relaxed mutant, no significant changes in the overall composition of the fatty acids were observed after the onset amino acid limitation. These results indicate that a change in fatty acid composition of membrane lipids after starvation of cells may be responsible for the prevention of loss of cellular proteins into the culture medium in stringent controlled cells of Escherichia coli. |
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