Regular proteinaceous layers ofThermococcus stetteri cell envelope |
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| Authors: | Dr Georgy M Gongadze Alla S Kostyukova Margarita L Miroshnichenko Elizaveta A Bonch-Osmolovskaya |
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| Institution: | (1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142292 Pushchino Moscow region, Russia;(2) Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow region, Russia;(3) Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia |
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| Abstract: | Proteinaceous layers of theThermococcus stetteri cell envelope were investigated and found to consist of regularly arrayed subunits 18 nm in diameter. According to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two major proteins were present. They were glycosylated and had molecular weights of 80,000 and 210,000. In addition to two external regular proteinaceous layers, cells ofT. stetteri were found to have internal regular layers tightly attached to the cytoplasmic membrane. In the region of flagella attachment to the cell, polar membrane-like structures were found in the cytoplasm. |
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