Characterization and biobleaching effect of hemicellulases produced by thermophilic fungi |
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Authors: | J Haarhoff CJ Moes C Cerff WJ van Wyk G Gerischer BJH Janse |
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Institution: | (1) Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600, South Africa;(2) Mondi Forests, Hilton , 3245, South Africa |
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Abstract: | The culture supernatant of Thermomyces lanuginosus strains MED 2D and MED 4B1 had high activities of xylanase with low inducible activities of -xylosidase. The crude xylanase was optimally active at 70 °C and at pH 6.0 to 6.5. Subsequently Eucalyptus kraft pulp was treated with MED 2D supernatant at 10 IU per gram pulp resulting in a 10.5% reduction in Kappa number. XECEDED-Refined bleached pulp resulted in handsheets with increased brightness compared to the control (X = xylanase treatment; E = alkaline extraction; C = Cl2 treatment; D = ClO2 treatment). |
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Keywords: | biobleaching hemicellulases Thermomyces lanuginosus thermophiles |
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