A temperature upshift induces initiation of replication at oriC on the Escherichia coli chromosome |
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Authors: | Emilia Botello,& Alfonso Jimé nez-Sá nchez |
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Affiliation: | Department of Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, E-06080 Badajoz, Spain. |
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Abstract: | A temperature upshift of 10 or more degrees in the growth temperature of a bacterial culture causes induction of extra rounds of chromosome replication. This heat-induced replication (HIR) initiates at oriC , is transitory, requires RNase H1 and RecA proteins and requires neither RNA polymerase activity nor de novo protein synthesis. The number of origins activated by heat is growth rate and temperature differential dependent. An origin activation higher than 20% increases the DNA:mass ratio around twofold, and this value is kept constant for the subsequent generations of growth at 41°C. We have also shown that HIR is neither related to SDR nor induced by the heat shock response. We suggest that a thermodynamic alteration of oriC structure or of membrane fluidity could explain the observed HIR. |
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