Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells |
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| Authors: | D Ghigo D Geromin C Franchino R Todde C Priotto C Costamagna M Arese G Garbarino G P Pescarmona A Bosia |
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| Institution: | (1) Dipartimento di Genetica, Biologia e Chimica Medica — Sezione di Chimica Medica, Via Santena 5/bis, I-10126 Torino, Italy;(2) Institute of Internal Medicine, University of Torino, Via Santena 5/bis, I-10126 Torino, Italy |
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| Abstract: | Summary The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5–8 times higher than human cells. Ionomycinelicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion. |
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| Keywords: | Amino acids Nitric oxide Endothelial cells Glutathione Polyomavirus N-(2-Mercaptopropionyl)-glycine 1-Chloro-2 4-dinitrobenzene |
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