Dielectric spectroscopy of Schizosaccharomyces pombe using electrorotation and electroorientation |
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| Affiliation: | 1. Research Group (Environmental Health), Sumitomo Chemical. Ltd, Hyogo 665-8555, Japan;2. Graduate School of Science and Engineering, Ritsumeikan University, Shiga 525-8577, Japan;3. University of Engineering and Technology, Vietnam National University, Hanoi, Vietnam;4. School of Mechanical and Electrical Engineering, University of Southern Queensland, Australia;1. Biomedical Engineering Department, Helwan University, Cairo, Egypt, Center of Nanoelectronics and Devices (CND), Zewail City of Science and Technology, The American University in Cairo (AUC), Cairo, Egypt;2. Department of Electronics and Communication Engineering, Misr International University, Cairo, Egypt, Center of Nanoelectronics and Devices (CND) at Zewail City for Science and Technology, The American University in Cairo (AUC), Cairo, Egypt;3. Graduate Program of Biotechnology, School of Sciences and Engineering, American University in Cairo (AUC), Cairo, Egypt;4. Biology Department, School of Sciences and Engineering, American University in Cairo (AUC), Cairo, Egypt, University of Medicine and Health Sciences, Saint Kitts and Nevis;5. Electronics and Communication Department, Kuwait College of Science and Technology, Kuwait, Biomedical Engineering Department, Helwan University, Cairo, Egypt, Center of Nano electronics and Devices (CND), Zewail City of Science and Technology, The American University in Cairo (AUC), Cairo, Egypt;6. Center of Nano electronics and Devices (CND), Zewail City of Science and Technology, The American University in Cairo (AUC), Cairo, Egypt |
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| Abstract: | Two complementary AC electrokinetic techniques electrorotation (ER) and electroorientation (EO) enabled the dielectric characterization of the rod-shaped fission yeast Schizosaccharomyces pombe. The use of microstructured electrodes allowed both ER and EO measurements to be performed over wide ranges of field frequency and medium conductivity. Due to their layered structure, living S. pombe cells exhibited up to three well resolved peaks in their ER spectra and also two distinct orientations, i.e., parallel or perpendicular to the imposed linear field. Heat treatment and enzymatic protoplast isolation led to dramatic changes in the electrokinetic behavior of fission yeast. Application of the theoretical models linking the ER and EO spectra yielded the dielectric parameters of the major structural units of S. pombe cells (cell wall, plasma membrane and cytosol). The dielectric characterization of yeasts has an enormous impact in biotechnology and biomedicine, because electric field pulse techniques (electrofusion and electropermeabilization) are widely used for production of transgenic yeast strains of economic importance. The present study also showed that combined ER and EO measurements can be employed as a powerful diagnostic tool for analyzing changes in yeast structure and physiology upon exposure to various stress conditions. |
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