Assessment of [18F]fluoroethylflumazenil metabolites using high-performance liquid chromatography and tandem mass spectrometry |
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| Affiliation: | 1. Unité de Chimie Pharmaceutique et de Radiopharmacie, Université Catholique de Louvain, Avenue Mounier 73.40, B-1200 Brussels, Belgium;2. Laboratoire de Spectrometrie de Masse, Université Catholique de Louvain, Place Pasteur 1, B-1348 Louvain-la-Neuve, Belgium;3. Unité de Tomographie par Positrons, Université Catholique de Louvain, Chemin du Cyclotron 2, B-1348 Louvain-la-Neuve, Belgium;4. Laboratoire de Résonance Magnétique Biomédicale, Université Catholique de Louvain, Avenue Hippocrate 10, B-1200 Brussels, Belgium;1. Department of Chemistry, The University of Technology, 35959 Rzeszow, Poland;2. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA;1. Division of Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, United States;2. Department of Molecular and Medical Pharmacology, University of California, Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, United States;3. Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju 561-712, Republic of Korea;4. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095, United States;1. Department of Physical Chemistry, University of Seville, C/ Profesor García González 1, 41012 Seville, Spain;2. NMR Service, University of Seville, Apartado 1203, E-41071 Seville, Spain;3. Department of Chemical Engineering, Physical Chemistry and Material Science, Faculty of Experimental Sciences, Campus El Carmen, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, Spain |
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| Abstract: | A simple procedure using HPLC and tandem mass spectrometry has been developed for the determination of fluoroethylflumazenil metabolites. Samples were precipitated with acetonitrile, evaporated to dryness followed by reconstitution with methanol. As mobile phase, 50 mM ammonium formate–methanol (58:42, v/v) was used. The method is valid both for cold and radiolabelled metabolites. Various cold metabolites (hydroxylated and/or dealkylated) were identified in rat and human microsome preparations. Radiolabelled metabolites arise from two or more transformations including hydroxylation. The methodology developed can be applied for further characterisation of metabolites, and for the determination of non metabolised [18F]fluoroethylflumazenil in routine clinical analysis. |
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