Simultaneous analysis of homocysteine and methionine in plasma |
| |
| Affiliation: | 1. Dionex Corporation, 445 Lakeside Drive, Sunnyvale, CA 94086, USA;2. The Banting Institute, University of Toronto, 100 College Street, Toronto, Ontario, Canada M5G1L5;1. Berlin University of Technology, Institute of Biotechnology, Berlin, Germany;2. Research and Teaching Institute for Brewing in Berlin, Department Bioprocess Engineering and Applied Microbiology, Berlin, Germany;1. Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt;2. Applied Nucleic Acids Research Center, Chemistry Department, Faculty of Science, Zagazig University, Egypt;3. Biochemistry Department, Faculty of Agriculture, Zagazig University, Egypt;1. Department of Occupational and Environmental Health, Wuhan 430030, Hubei, PR China;2. Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, PR China;1. Faculty of Health Sciences, Universiti Sultan Zainal Abidin, 21300 Kuala Terengganu, Malaysia;2. School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;3. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia |
| |
| Abstract: | The new isocratic cation exchange method separates up to eight different amino thiols. The separated sample components are detected electrochemically using a gold electrode and the integrated pulsed amperometry. The eluent composition is, for example, 0.15 M sodium perchlorate, 0.02 M perchloric acid and 5% acetonitrile. The report describes the optimization of chromatographic parameters such as column diameter and eluent composition. Quantitative performance is discussed for eight different amino thiols using standards. Also presented is a long term quantitative study for homocysteine and methionine in plasma samples. The preparation of plasma samples is simpler than with the previously reported version of the method. Only a reduction step is required, and neither column switching nor derivatization are necessary. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
