High-performance liquid chromatographic determination for bile components in fish,chicken and duck |
| |
| Institution: | 1. Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence, “Campus Mare Nostrum”, University of Murcia, Murcia, Spain;2. Department of Public Health, Sanitary and Consumption Office, Murcia Town Council, Ronda de Levante 11, 30008 Murcia, Spain;1. Department of Internal Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands;2. Diabetes Center, Department of Internal Medicine, VU University Medical Center, Amsterdam, The Netherlands;3. Department of Endocrinology and Metabolism, Academic Medical Center, Amsterdam, The Netherlands;4. Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands |
| |
| Abstract: | A HPLC procedure for the determination of 13 bile acids and cyprinol sulfate in animals was developed. The mobile system 0.3% ammonium carbonate solution–acetonitrile (73:27, v/v) 10 min→(68:32) 10 min→(50:50) 10 min was available for separating all 14 bile components, except for deoxycholic and glycodeoxycholic acids, which could be further separated with 0.3% ammonium carbonate solution–acetonitrile (73:27). After applying this method, grass carp and common carp bile was found to contain mainly cyprinol sulfate, while the other 12 fish species bile contained mainly taurocholic, taurochenodeoxycholic and cholic acids. Chicken bile was mainly composed of glycolithocholic and taurocholic acids, but duck bile was mainly composed of taurochenodeoxycholic, cholic and ursodeoxycholic acids. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
