Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography |
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| Institution: | 1. Clinic of Digestive Surgery, Clinical Center of Serbia, Belgrade, Serbia;2. Faculty of Medicine, University of Belgrade, Serbia;3. Clinic of Gastroenterology and Hepatology, Clinical Center of Serbia, Belgrade, Serbia;4. Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia;5. Center for Medical Biochemistry, Clinical Center of Serbia, Belgrade, Serbia;6. Institute of Pathology, Belgrade, Serbia;7. Institute of Medical Statistics and Informatics, Belgrade, Serbia;8. Clinic of Hematology, Clinical Center of Serbia, Belgrade, Serbia;9. Karolinska Institute, Department of Medicine Solna, Rheumatology Unit and Karolinska University Hospital, Rheumatology Clinic, Stockholm, Sweden;1. Dept. of Anaesthesiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands;2. Dept. of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Hospital Frankfurt, Frankfurt/Main, Germany |
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| Abstract: | Estimation of lipid peroxidation through MDA formation measured by assaying thiobarbituric acid (TBA) reactive products separated by HPLC remains the method of choice to study the development of oxidative stress in blood plasma. In this report we describe the influence of citrate and EDTA anticoagulants used for blood collection on estimation of MDA concentrations using HPLC analysis of MDA-TBA adducts. We analyzed a group of 40 blood donors (21 men and 19 women), median age 27 years, range 19–48 years. The mean MDA concentration in citrate plasma was 1.43±0.51 μmol/l (range: 0.61–2.57 μmol/l) and in EDTA plasma 0.36±0.10 μmol/l (range: 0.13–0.63 μmol/l). There was a significant difference in MDA mean concentration that we attribute to different antioxidant properties of anticoagulants used for blood collection. Consistency in the choice of anticoagulant is clearly extremely important. |
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