Morphology and dynamics of chromosome territories in living cells |
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| Institution: | 1. Kirchhoff Institute for Physics, University of Heidelberg, Albert-Ueberle-Str. 3–5, 69120 Heidelberg, Germany;2. Interdisciplinary Center of Scientific Computing, University of Heidelberg, INF 368, 69120 Heidelberg, Germany;3. Institute of Anthropology and Human Genetics, LMU Munich, Goethestr. 31, 80336 Munich, Germany;4. Institute of Anthropology and Human Genetics, LMU Munich, Richard-Wagner-Str. 10/I, 80333 Munich, Germany;1. Stabsstelle Ärztlicher Leiter Rettungsdienst, Feuerwehr, Stadt Braunschweig, Deutschland;2. Fakultät für Gesundheitswissenschaften, Ostfalia Hochschule, Wolfsburg, Deutschland;3. Institut für Allgemeinmedizin, Medizinische Hochschule Hannover, Deutschland;4. Gesundheitsamt, Stadt Braunschweig, Deutschland;5. Sozialreferat, Stadt Braunschweig, Deutschland;6. Peter L. Reichertz Institut für Medizininformatik, Braunschweig, Deutschland;7. Abteilung Allgemeinmedizin & Versorgungsforschung, Medizinische Fakultät Heidelberg, Deutschland;1. Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston;3. Divison of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston;2. Dalio Institute of Cardiovascular Imaging, New York-Presbyterian Hospital and Weill Cornell Medical College, New York;4. Emergency Medicine, Department of Medical-Surgery Sciences and Translational Medicine, University Sapienza Rome, Sant''Andrea Hospital, Italy;1. Institute of Low Temperature Science, Hokkaido University, N19W8, Kita-ku, Sapporo 060-0819, Japan;2. Department of Natural History Sciences, Faculty of Science, Hokkaido University, N10W8, Kita-ku, Sapporo 060-0810, Japan;1. Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18, E-08034 Barcelona, Spain;2. Barcelona Center for Subsurface Imaging (Barcelona-CSI-CSIC), Passeig Marítim de la Barceloneta 37, E-08003 Barcelona, Spain;1. Asan Institute for Life Sciences, University of Ulsan, College of Medicine, Asan Medical Center, Seoul 138-736, Republic of Korea;2. Dept. of Pathology, College of Dentistry, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea |
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| Abstract: | Chromosome territories formed by fluorescence-labeled sub-chromosomal foci were analyzed in time-lapse series of 3D confocal data sets of living HeLa and human neuroblastoma cells. The quantitative analysis of the chromosome territory morphology confirmed previous results obtained by visual observation Zink et al., Hum. Genet. 102 (1998) 241–251] that chromosome territories persisted as stable entities over an observation time >4 h. The changes in morphology with time of single chromosome territories were found to be less pronounced than differences in morphology of different chromosome territories in fixed cells. The analysis of the individual motion of chromosome territories recently showed ‘Brownian’ diffusion-like motion at very slow rates Bornfleth et al., Biophys. J. 77 (1999) 2871–2886]. Here, we show that the mutual motion of different chromosome territories was independent and also ‘Brownian’ diffusion-like. |
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