High-performance liquid chromatography with electrochemical detection for the determination of 7-monohydroxyethylrutoside in plasma |
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| Affiliation: | 1. Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands;2. Division of Human Toxicology, University of Maastricht, Maastricht The Netherlands;1. Institute of Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 159, Prosp. 100-letiya Vladivostoka, Vladivostok 690022, Russia;2. Institute of Problems of Chemical Physics, Russian Academy of Sciences, 1, Prosp. akad. Semenova, Chernogolovka, Moscow Region 142432, Russia;1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China;2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China;1. Department of Ecological and Sustainable Production Systems, Faculty of Horticultural Science, Szent István University, 29-43 Villányi út, H-1118 Budapest, Hungary;2. Department of Postharvest Sciences and Sensory Evaluation, Faculty of Food Science, Szent István University, 29-43 Villányi út, H-1118 Budapest, Hungary;3. Department of Dietetics and Nutrition Science, Faculty of Health Sciences, Semmelweis University, 17 Vas utca, H-1088 Budapest, Hungary;4. Department of Research Methodology and Statistics, Institute of Sectorial Economics and Methodology, Faculty of Economics and Business, University of Debrecen, 138. Böszörményi út, H-4032 Debrecen, Hungary |
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| Abstract: | MonoHER (7-monohydroxyethyl rutoside) is a semisynthetic flavonoid, which can be used as a modulator for doxorubicin-induced cardiotoxicity. To study the pharmacokinetics of monoHER in mice and human an HPLC procedure was developed to measure the level of monoHER in plasma. After extraction of monoHER with methanol, the supernatant was equally diluted (v/v) with 25 mM phosphate buffer (pH 3.33). This solution was analysed by HPLC, using a reversed-phase ODS column, with a mobile phase consisting of 49% methanol and 51% of an aqueous solution containing 10 mM sodium dihydrogen phosphate (pH 3.4), 10 mM acetic acid and 36μM EDTA. The retention time of monoHER was about 5.2 min. The lower limit of quantification of monoHER was set at 0.3 μM and the calibration line was linear up to 75 μM. The within-day accuracy and precision of the quality control samples (0.45, 1.0, 10 and 40 μM) were better than 15 and 13%, respectively. The between-day accuracy and precision were less than 3, 20%, respectively. The recovery of monoHER (using quality control concentrations) was concentration independent and ranged from 90.5 to 95.3% except for the lowest quality control, 0.45 μM, of which the recovery was 85%. The concentration of monoHER in plasma decreased with 10% when stored at −80°C for one month and with 20% when stored at −20°C for 3 weeks. The repeated injection of monoHER in aliquots of 10 μM, stored in the autosampler tray (4°C), showed a consistent decrease during a run: 15% over 24 h. To compensate for this decrease, sample duplicates were analysed in a mirror image sequence. |
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