Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus |
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| Affiliation: | 1. Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milano, Italy;2. Department of Health, Animal Science and Food Safety, University of Milan, via Celoria 10, 20133 Milano, Italy;1. Christian Doppler Laboratory for Immunomodulation, 1090 Vienna, Austria;2. Institute of Immunology, Medical University of Vienna, 1090 Vienna, Austria;3. Department of Laboratory Medicine, Medical University of Vienna, 1090 Vienna, Austria;4. Department of Medicine, University of Tennessee Health Science Center, Memphis, 38163, TN, USA;5. Memphis Veterans Affairs Medical Center, 38104, TN, USA;6. Department of Pathology, University of Tennessee Health Science Center, Memphis, 38163, TN, USA;7. Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany;8. Department of Laboratory Animal Pathology, Medical University of Vienna, 1090 Vienna, Austria;9. Department of Laboratory Animal Pathology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;10. Ludwig Boltzmann Institute for Cancer Research, Vienna, Austria;11. Department of Pathophysiology and Allergy Research, Medical University of Vienna, 1090 Vienna, Austria |
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| Abstract: | In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10–15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Physiol. Scand. 126 (1986) 601–607]. The enzyme could be detected in the intestinal epithelial cells by immunohistochemistry. Furthermore, immunoreactive intact EFE-III-1 was found in serum or plasma after intraperitoneal injection of rats. Approx. 10% of the full-size enzyme could transport through the intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection. |
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