Determination of fluvastatin and its five metabolites in human plasma using simple gradient reversed-phase high-performance liquid chromatography with ultraviolet detection |
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| Institution: | 1. Tsukuba Research Institute, Novartis Pharma K.K., Ohkubo 8, Tsukuba-shi, Ibaraki 300-2611, Japan;2. Chemicals Evaluation and Research Institute, 19–14, Chuo-machi, Kurume-shi, Fukuoka 830-0023, Japan;1. Center of Studies in Health and Environment, School of Science and Technology, National University of San Martín, San Martín, Buenos Aires, Argentina;2. Department of Pharmaceutical Science, Hampton University, VA, USA;3. Department of Histology and Embryology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina;1. Department of Forensic Pathology, College of Forensic Medicine, Xi''an Jiaotong University, Xi’an 710061, China;2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China;3. Department of Biochemistry and Physiology, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China;1. Department of Chemistry, Bohai University, Jinzhou 121013, China;2. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China |
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| Abstract: | A simple gradient reversed-phase high-performance chromatographic method with ultraviolet detection for the determination of fluvastatin (FV) and its five metabolites, (M-2, M-3, M-4, M-5 and M-7) in human plasma was developed and validated. The limit of quantification of FV and its five metabolites in human plasma was 10 ng ml−1. The assay had satisfactory selectivity, recovery, linearity and precision accuracy. Stability studies showed that FV and its five metabolites were stable in plasma up to at least 1 month of storage at −30°C. |
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