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Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine
Affiliation:1. Department of Chemistry, Chandigarh University Mohali, India;2. Department of Mechanical Engineering, GLA University Mathura, 281406, India;1. Key Laboratory of Aerospace Advanced Materials and Performance of Ministry of Education, School of Materials Science & Engineering, Beihang University, Beijing 100191, China;2. School of Chemical and Environmental Engineering, Anyang Institute of Technology, Anyang 455000, China;1. Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Napoli, Italy;2. Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università di Napoli Federico II, Piazzale Tecchio 80, 80125 Napoli, Italy;3. Istituto di Ricerche sulla Combustione, IRC-CNR, Piazzale Tecchio 80, 80125 Napoli, Italy;1. Department of Medical Physics, Anna University, Chennai 600025, India;2. Department of Oral Medicine and Radiology, Meenakshi University, Chennai 600095, India;3. Barnard Institute of Radiology, Madras Medical College, Chennai 600006, India
Abstract:A high-performance liquid chromatographic method for the determination of ofloxacin in human plasma and urine was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm. The assay was linear from 0.5 to 10.0 μg/ml. The relative standard deviation of intra- and inter-day assays was lower than 5%. The average recovery of ofloxacin from plasma was 93%. The method was evaluated in samples from healthy subjects whose drug levels were already measured by microbiological assay.
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