Biosurfactant production and hydrocarbon-degradation by halotolerant and thermotolerant Pseudomonas sp. |
| |
Authors: | Manoj Kumar Vladimir León Angela De Sisto Materano Olaf A. Ilzins Luis Luis |
| |
Affiliation: | (1) Unidad de Biotecnología del Petróleo, Centro de Biotecnología, Fundación Instituto de Estudios Avanzados (IDEA), Apartado 17606, Caracas, 1015 A, Venezuela;(2) Indian Oil Corporation, Research and Development Center, Faridabad, 121007, Haryana, India |
| |
Abstract: | A hydrocarbon degrading and biosurfactant producing, strain DHT2, was isolated from oil-contaminated soil. The organism grew and produced biosurfactant when cultured in variety of substrates at salinities up to 6 g l−1 and temperatures up to 45°C. It was capable of utilizing crude oil, fuels, alkanes and PAHs as carbon source across the wide range of temperature (30–45°C) and salinity (0–6%). Over the range evaluated, the salinity and temperature did not influence the degradation of hydrocarbon and biosurfactant productions. Isolate DHT2 was identified as Pseudomonas aeruginosa by analysis of 16S rRNA sequences (100% homology) and biochemical analysis. PCR and DNA hybridization studies revealed that enzymes involved in PAH metabolism were related to the naphthalene dioxygenase pathway. Observation of both tensio-active and emulsifying activities indicated that biosurfactants were produced by DHT2 during growth on both, water miscible and immiscible substrates, including PAH. The biosurfactants lowered the surface tension of medium from 54.9 to 30.2 dN/cm and formed a stable emulsion. The biosurfactant produced by the organism emulsified a range of hydrocarbons with hexadecane as best substrate and toluene was the poorest. These findings further indicate that the isolate could be useful for bioremediation and bio-refining application in petroleum industry. |
| |
Keywords: | Pseudomonas sp. Biosurfactant Halotolernat Themotolerant Hydrocarbon Biodegradation |
本文献已被 SpringerLink 等数据库收录! |
|