ICR 小鼠自然感染小鼠肝炎病毒后抗原抗体的变化

目的：了解小鼠肝炎病毒（ MHV）污染的设施内，ICR小鼠自然感染后MHV抗原抗体存在情况。方法选择50只ICR小鼠，通过更换“脏垫料”的方式进行饲养，分别在实验第2，4，8，14，21，28，35，42，56和84天各剖杀动物5只，采集血清、盲肠内容物、粪便、肝脏以及肺脏检测抗原抗体分布情况。结果实验开始第2天，肺脏中检出小鼠肝炎病毒，检出率为20％（1/5），第4天开始，肝脏、肺部、盲肠以及粪便中均可检出小鼠肝炎病毒；84天后，肺脏、盲肠、粪便和肝脏阳性检出率降为0。实验第8天，血清中抗体检出阳性，阳性率为100％（5/5），直至第84天实验结束，抗体阳性率仍维持在100％（5/5）。结论血清学方法可作为日常监督主要诊断方法，而抗原检测方法只能应用于早期诊断。

Dynamic changes of viral loads and antibody responses in ICR mice naturally infected with hepatitis virus

Objective To define dynamic changes of viral loads and antibody responses in ICR mice naturally infected with hepatitis virus in an MHV contaminative facility .Method A total 50 ICR were housed by different “dirty bedding” exposure.Antigen and antibody was detected after 2,4,8,14,21,28,35,42,56 and 84 days.Result Mouse hepatitis virus （MHV） was detected in lung after 2 days, and positive rate is 20% （1/5）.MHV was detected in lung, liver, cecal and feces during 4 and 56 days.The positive rate was 0/5 in lung, liver, cecal and feces on 84 days after experiments.Antibody positive rates were 100%during 8 and 84 days.Conclusion Serological method can be used as the main method for the diagnosis of the daily supervision , and antigen detection method can only be applied to early diagnosis .