DNA isolation from fresh,dry plant samples with highly acidic tissue extracts |
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| Authors: | Dnyaneshwar Warude Preeti Chavan Kalpana Joshi Bhushan Patwardhan |
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| Institution: | (1) Interdisciplinary School of Health Sciences, University of Pune, Ganeshkhind, 411 007 Pune, India |
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| Abstract: | Current DNA isolation methods are limited in their ability to obtain quality and/or quantity DNA from plants, such asEmblica officinalis, Terminalia belerica, andTerminalia chebula, which have low pH and high amounts of secondary metabolites in tissue extracts. Our modified DNA isolation method yields
good-quality, high-molecular-weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification.
This method is also useful for isolating DNA from dry powders. |
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| Keywords: | DNA isolation Emblica officinalis low pH PCR Terminalia belerica Terminalia chebula triphala churna |
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