Modulation of sulfur metabolism enables efficient glucosinolate engineering |
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| Authors: | Morten E Møldrup Fernando Geu-Flores Carl E Olsen Barbara A Halkier |
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| Institution: | (1) Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;(2) VKR Research Centre for Pro-Active Plants, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;(3) Department of Natural Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark |
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| Abstract: | Background Metabolic engineering in heterologous organisms is an attractive approach to achieve efficient production of valuable natural
products. Glucosinolates represent a good example of such compounds as they are thought to be the cancer-preventive agents
in cruciferous plants. We have recently demonstrated that it is feasible to engineer benzylglucosinolate (BGLS) in the non-cruciferous
plant Nicotiana benthamiana by transient expression of five genes from Arabidopsis thaliana. In the same study, we showed that co-expression of a sixth Arabidopsis gene, γ-glutamyl peptidase 1 (GGP1), resolved a metabolic bottleneck, thereby increasing BGLS accumulation. However, the accumulation did not reach the expected
levels, leaving room for further optimization. |
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