A systematic investigation of <Emphasis Type="Italic">Escherichia coli</Emphasis> central carbon metabolism in response to superoxide stress |
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| Authors: | Bin Rui Tie Shen Hong Zhou Jianping Liu Jiusheng Chen Xiaosong Pan Haiyan Liu Jihui Wu Haoran Zheng Yunyu Shi |
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| Institution: | (1) Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 230026 Hefei, China;(2) Department of Computer Science and Technology, University of Science and Technology of China, 230026 Hefei, China |
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| Abstract: | Background The cellular responses of bacteria to superoxide stress can be used to model adaptation to severe environmental changes. Superoxide
stress promotes the excessive production of reactive oxygen species (ROS) that have detrimental effects on cell metabolic
and other physiological activities. To antagonize such effects, the cell needs to regulate a range of metabolic reactions
in a coordinated way, so that coherent metabolic responses are generated by the cellular metabolic reaction network as a whole.
In the present study, we have used a quantitative metabolic flux analysis approach, together with measurement of gene expression
and activity of key enzymes, to investigate changes in central carbon metabolism that occur in Escherichia coli in response to paraquat-induced superoxide stress. The cellular regulatory mechanisms involved in the observed global flux
changes are discussed. |
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