Enhanced welan gum production using a two-stage agitation speed control strategy in Alcaligenes sp. CGMCC2428 |
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Authors: | Hui Li Hong Xu Hao Xu Sha Li Han-Jie Ying Ping-Kai Ouyang |
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Institution: | (1) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, Nanjing, 210009, China;(2) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, National Engineering Technique Research Center for Biotechnology, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, Nanjing, 210009, China; |
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Abstract: | Batch fermentative production of welan gum by Alcaligenes sp. CGMCC2428 was investigated under various oxygen supply conditions using regulating agitation speed. Based on a three
kinetic parameters analysis that includes specific cell growth rate (μ), specific glucose consumption rate (q
s), and specific welan formation rate (q
p), a two-stage agitation speed control strategy was proposed to achieve high concentration, high yield, and high viscosity
of welan. During the first 22 h, the agitation speed in 7.5 L fermenter was controlled at 800 rpm to maintain high μ for cell growth. The agitation was then reduced step-wise to 600 rpm to maintain a changing profile with stable dissolved
oxygen levels and obtain high qp for high welan accumulation. Finally, the maximum concentration of welan was reached at 26.3 ± 0.89 g L−1 with a yield of 0.53 ± 0.003 g g−1 and the welan gum viscosity of 3.05 ± 0.10 Pa s, which increased by an average of 15.4, 15.2, and 20.1% over the best results
controlled by constant agitation speeds. |
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Keywords: | |
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