Contribution of the FtsQ transmembrane segment to localization to the cell division site |
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Authors: | Scheffers Dirk-Jan Robichon Carine Haan Gert Jan den Blaauwen Tanneke Koningstein Gregory van Bloois Edwin Beckwith Jon Luirink Joen |
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Institution: | Molecular Microbiology, Institute for Molecular Cell Biology, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands. dirk-jan.scheffers@falw.vu.nl |
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Abstract: | The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. |
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