Collagenase production by immortalized human aortic endothelial cells infected with simian virus 40 |
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Authors: | Yasuyuki Sasaguri Hidetaka Yanagi Hideaki Nagase Ryuji Nakano Shyuichi Fukuda Minoru Morimatsu |
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Institution: | 1. Department of Pathology, Kurume University School of Medicine, 830, Kurume, Japan 2. Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, 11603, Kansas City, KS, USA
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Abstract: | Human aortic endothelial cells, isolated at autopsy from a 52-year-old male dying from lung cancer, were treated with simian
virus 40 (SV40). One colony was isolated from the infected endothelial cell culture 4 weeks after infection. The cells expressed
SV40 large T antigen and p53 protein (p53) in their nuclei but lacted the characteristics of a transformed phenotype. The
cells grew well in a monolayer over the 97th passage and exhibited Factor Vlll-related antigen, Ulex europaeus 1 agglutinin
(UEA-1) as endothelial cell markers, and a well-developed fibronectin network. The amount of prostacyclin synthesized by the
cells was less than the amount synthesized by normal aortic or umbilical cord vein endothelial cells. The cells produced relatively
large amounts of procollagenase, and 12-o-tetradecanoyl-phorbol-13-acetate (TPA) augmented the ability of the cells to produce
this enzyme. These immortalized human aortic endothelial cells, which have some characteristics of normal endothelial cells
and, like capillary endothelial cells, have the ability to produce collagenase, will probably prove useful for studies of
atherosclerosis and angiogenesis. |
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Keywords: | Human aortic endothelial cell Immortalization Simian virus 40 Variant endothelial cell Collagenase |
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