首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Collagenase production by immortalized human aortic endothelial cells infected with simian virus 40
Authors:Yasuyuki Sasaguri  Hidetaka Yanagi  Hideaki Nagase  Ryuji Nakano  Shyuichi Fukuda  Minoru Morimatsu
Institution:1. Department of Pathology, Kurume University School of Medicine, 830, Kurume, Japan
2. Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, 11603, Kansas City, KS, USA
Abstract:Human aortic endothelial cells, isolated at autopsy from a 52-year-old male dying from lung cancer, were treated with simian virus 40 (SV40). One colony was isolated from the infected endothelial cell culture 4 weeks after infection. The cells expressed SV40 large T antigen and p53 protein (p53) in their nuclei but lacted the characteristics of a transformed phenotype. The cells grew well in a monolayer over the 97th passage and exhibited Factor Vlll-related antigen, Ulex europaeus 1 agglutinin (UEA-1) as endothelial cell markers, and a well-developed fibronectin network. The amount of prostacyclin synthesized by the cells was less than the amount synthesized by normal aortic or umbilical cord vein endothelial cells. The cells produced relatively large amounts of procollagenase, and 12-o-tetradecanoyl-phorbol-13-acetate (TPA) augmented the ability of the cells to produce this enzyme. These immortalized human aortic endothelial cells, which have some characteristics of normal endothelial cells and, like capillary endothelial cells, have the ability to produce collagenase, will probably prove useful for studies of atherosclerosis and angiogenesis.
Keywords:Human aortic endothelial cell  Immortalization  Simian virus 40  Variant endothelial cell  Collagenase
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号