Modified Pagano Levin Medium to Isolate Candida Species

The use of Pagano Levin medium for primary isolation of Candida species from highly contaminated specimens proved unsatisfactory owing to overgrowth by bacteria and fungi. Addition of cycloheximide and chloramphenicol to the medium greatly improved results by inhibiting overgrowth. The additional inhibitors also altered rate of growth and degree of pigmentation reported for some of the Candida species.