| SDS-蛋白复合物的性质及其液相色谱分离研究 |
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| 引用本文： | 徐明波,姚志建. SDS-蛋白复合物的性质及其液相色谱分离研究[J]. 生物化学与生物物理进展, 1992, 19(3): 194-198 |
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| 作者姓名： | 徐明波 姚志建 |
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| 作者单位： | 军事医学科学院基础医学研究所;军事医学科学院基础医学研究所 |
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| 摘 要： | 利用一些常用的蛋白质液相色谱的分离方法,首次系统地研完了SDS-蛋白复合物的性质及其液相色谱分离规律,并对有关的原理进行了探讨。该项研究为膜蛋白及基因工程产生的包涵体蛋白的研究提供了重要的参数。
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| 关 键 词： | SDS-蛋白复合物 液相色谱分离 白介素-2 |
| 收稿时间： | 1991-01-28 |
| 修稿时间： | 1991-05-23 |
THE STUDY OF SDS-PROTEIN COMPLEX PROPERTY AND ITS LIQUID CHROMATOGRAPHIC SEPARATION |
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| Xu Mingbo and Yao Zhijian. THE STUDY OF SDS-PROTEIN COMPLEX PROPERTY AND ITS LIQUID CHROMATOGRAPHIC SEPARATION[J]. Progress In Biochemistry and Biophysics, 1992, 19(3): 194-198 |
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| Authors: | Xu Mingbo and Yao Zhijian |
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| Affiliation: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences;Institute of Basic Medical Sciences, Academy of Military Medical Sciences |
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| Abstract: | A systematic investigation of the property of the SDS-protein complex and the influence of the detergent sodium doedecyl sulphate (SDS) on protein purification is reported. The data demonstrate that SDS in the sample doesn't disturb gel permeation and hydro xyapatite chromatographic separation, but reduces the separation efficiency and protein recovery in hydrophobic interaction, affinity and reversed-phased chromatographic methods. A successful separation of many membrane and inclusion body proteins in genetic engineering will therefore require new concepts in column design as well as critical revision of the choice of mobile phases. This study provides important data on membrane and inclusion body proteins purification. |
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| Keywords: | SDS-protein complex chromatography protein purification interleukin-2 |
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