Steroid transformation using magnetically immobilized Mycobacterium sp. |
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Affiliation: | 1. Univ. Grenoble Alpes, CNRS, LIPhy, 38000 Grenoble, France;2. Laboratoire de Spectroscopie et Dynamique Moléculaire, Ecole Nationale Supérieure d''Ingénieurs de Tunis, Université de Tunis, 5 Av Taha Hussein 1008 Tunis, Tunisia;3. GSMA, UMR CNRS 7331, University of Reims Champagne Ardenne, Moulin de la Housse B.P. 1039, Cedex Reims F-51687, France |
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Abstract: | Mycobacterium sp. (NRRL-B 3683) has been immobilized by adhesion of magnetic materials of submicron size to the bacterial surface. Preparations based on laboratory-prepared magnetic oxide that had been derivatized with hydrophobic octyltrichlorosilane showed the best properties. The magnetically immobilized bacteria were used for side-chain degradation of cholesterol into androsta-1,4-diene-3,17-dione. The magnetic bacteria behaved as free cells in the transformation media and no mass transfer limitations were observed. The magnetic bacteria could be used repeatedly without any cell loss, the cells being retrieved at the end of each transformation cycle by a magnet. |
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