Cellulase production by Talaromyces emersonii CBS 814.70 and a mutant UV7 during growth on cellulose,lactose and glucose containing media |
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Affiliation: | 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), Shanghai 200237, China;3. Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China |
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Abstract: | Recently it has been reported that Talaromyces emersonii CBS 814.70 is capable of growth on lactose containing media. During growth on such media large amounts of β-glucosidase are secreted into the medium. In addition, low levels of endoglucanase activity have been detected. In order to enhance endoglucanase production, u.v. irradiation and a modified selection procedure yielded a number of mutants. One of these, UV7, was capable of increased cellulase production during growth on cellulose, lactose and glucose containing media. Comparative studies between the wild-type organism and the mutant have shown that the former apparently produces constitutive levels of both endoglucanase and β-glucosidase. The form of β-glucosidase that appears to be constitutive is that form previously named BG-I. |
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