斯氏按蚊生长发育中唾液腺配子体激活因子的变化

为探讨斯氏按蚊生长发育过程中唾液腺抽提物配子体激活因子的消长,应用体外雄配子体出丝观察方法比较羽化后未吸血及吸血组雌性斯氏按蚊唾液腺抽提物中配子体激活因子对柏氏疟原虫雄配子体出丝诱导活性的动态变化。羽化后未吸血组按蚊唾液腺配子体激活因子活性与按蚊生长、发育呈同步变化。羽化后当日至羽化后第6 d,吸血组按蚊唾液腺抽提物的GAF活性变化与未吸血组相似,吸血后该组GAF活性下降,羽化后14 d恢复到吸血前水平。吸血后斯氏按蚊唾液腺配子体激活因子活性的降低与蚊卵发育可能相关。

Changes of Gametocyte Activating Factor in the Salivary Glands of Anopheles stephensi During Growth and Development

The dynamic changes of gametocyte activating factor(GAF) activity in the salivary gland extracts were compared between non-blood-fed and blood-fed male Anopheles stephensi post-emergence by in vitro exflagellation assay of Plasmodium berghei in order to investigate the rise and fall of GAF in the salivary glands of Anopheles stephensi during growth and development.After emergence,the varying tendency of GAF activity in the salivary glands of non-blood-fed mosquitoes was consistent with their growth and development.On the day 0 to day 6 after emergence,GAF activity in the salivary glands of blood-fed mosquitoes was similar to that of non-blood-fed mosquitoes,it declined after blood feeding,and recovered to the original level of pre-feeding on day 14 after emergence.The fall of GAF activity in the anopheline salivary glands after blood feeding may be related to development of mosquito eggs.