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Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns |
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| Authors: | Monique Burrus Jean Molinier Christophe Himber Reiner Hunold Roberte Bronner Philippe Rousselin Günther Hahne |
| Institution: | (1) Institut de Biologie Moléculaire des Plantes, CNRS, 12, Rue du Général Zimmer, 67084 Strasbourg Cedex, France;(2) Université Louis Pasteur, 12, Rue du Général Zimmer, 67084 Strasbourg Cedex, France;(3) Present address: Laboratoire de Biologie et Physiologie Végétales, UFR Sciences, Université de Reims-Champagne Ardenne, 51062 Reims Cedex, France;(4) Present address: Newlab, Max-Plack-Strasse 15a, 40699 Erkrath, Germany |
| Abstract: | Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme  -glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots. The results are consistent with the hypothesis that transformed shoots originate from transformation events that have occurred within the existing meristems. Two of the resulting chimaeric plants have been analysed in detail, and some representative gene integration patterns are presented. |
| Keywords: | chimaeric gene expression GUS expression Helianthus annuus meristems |
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