Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase |
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Authors: | Wittrup K D Weber A L Tsai P |
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Affiliation: | Department of Chemical Engineering, University of Illinois, Urbana, Illinois 61801. |
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Abstract: | We have developed a microencapsulation selection method which allows the rapid and quantitative screening of >10(6) yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400-fold single-pass enrichment for high-secreting mutants, and can be straightforwardly adapted for application to growth-based selection schemes with other microorganisms and enzymes. (c) 1993 John Wiley & Sons, Inc. |
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Keywords: | microencapsulation selection secretion yeast |
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