| pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用 |
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| 引用本文： | Liu D,Lu JS,Yin XL. pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用[J]. 生理学报, 2000, 52(6): 483-486 |
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| 作者姓名： | Liu D Lu JS Yin XL |
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| 作者单位： | 浙江大学医学院附属第二医院心内科,杭州,310006 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina (No 39770 319) |
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| 摘 要： | 观察ｐｐ６０ｃ－ｓｒｃ在血管紧张素Ⅱ（ＡｎｇⅡ）诱导血管平滑肌细胞（ＶＳＭＣｓ）内丝裂原活化蛋白激酶（ＭＡＰＫ）激活中的作用,以了解ＡｎｇⅡ促ＶＳＭＣｓ增殖的信号转导过程。将合成的反义ｃ－ｓｒｃ寡脱氧核苷酸（ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅ－ｏｔｉｄｅｓ,ＯＤＮｓ）以脂质体包裹转染培养的大鼠ＶＳＭＣｓ,用Ｗｅｓｔｅｒｎ印迹测得细胞裂解液中ｐｐ６０ｃ－ｓｒｃ含量明显下降,免疫沉淀方法测得ｐｐ６０ｃ－ｓ
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| 关 键 词： | 血管平滑肌细胞 丝裂原活化蛋白激酶 AngⅡ |
Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells |
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| Liu D,Lu J S,Yin X L. Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells[J]. Acta Physiologica Sinica, 2000, 52(6): 483-486 |
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| Authors: | Liu D Lu J S Yin X L |
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| Affiliation: | Department of Cardiology, The Second Affiliated Hospital to Zhejiang University Medical School, Hangzhou 310006. liuding99cn@yahoo.com.cn |
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| Abstract: | The effect of pp60c-src on mitogen-activated protein kinase (MAPK) activation induced by angiotensin II (Ang II) in vascular smooth muscle cells (VSMCs) was observed to explore the signal transduction process of VSMCs proliferation mediated by Ang II. Following transfection of the synthetic antisense c-src oligodeoxynucleotides (ODNs) wrapped in lipofectin into cultured rat VSMCs, pp60c-src content markedly decreased, pp60c-src kinase activity was only 14.4% of control, while MAPK activity was not significantly changed (P>0.05). When these transfected VSMCs were further stimulated by Ang II, the increase in MAPK activity was only 1.6% of control. These data suggest that pp60c-src is needed for MAPK activation in VSMCs stimulated by Ang II, and pp60c-src may be an important signaling molecule in abnormal proliferation of VSMCs. |
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| Keywords: | vascular smooth muscle cells mitogen activated protein kinase c src angiotensiL |
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