Chemical synthesis of versatile adaptors for molecular cloning. |
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Authors: | C P Bahl R Wu R Brousseau A K Sood H M Hsiung S A Narang |
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Abstract: | In this communication we report the chemical synthesis of two types of oligodeoxynucleotides to be used as adaptors in molecular cloning. The first type is used to create specific cohesive end sequences at the termini of a blunt-end DNA molecule without the use of restriction enzymes. The second type of adaptor is used to convert one kind of restriction-enzyme-generated specific cohesive end to another. This includes both the conversion of one type 5′-protruding end to another 5′-protruding end, and of a 3′- to a 5′-protruding end and vice versa. |
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Keywords: | To whom correspondence should be addressed |
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