Effects on in vitro brain protein synthesis of a translational inhibitor isolated from rabbit brain following intravenous administration of LSD |
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Authors: | Scott W Fleming Ian R Brown |
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Institution: | (1) Department of Zoology, University of Toronto Scarborough Campus, M1C 1A4 West Hill, Ontario, Canada |
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Abstract: | Inhibition of brain protein synthesis following intravenous administration of d-lysergic acid diethylamide (LSD) is accompanied by generation of a translational inhibitor protein in the postribosomal supernatant of cerebral hemispheres. Addition of an enriched preparation of this factor to a brain cell-free translation system resulted in a selective reduction in the level of phosphorylation of proteins of molecular weight 55K, 41K, and 25K. A similar set of changes was also observed in a brain cell-free system prepared 1 hr subsequent to drug injection. The brain inhibitor reduced the translational capacity of a messenger RNA-dependent reticulocyte lysate programmed with brain polysomes isolated from saline-injected animals however little effect was apparent when polysomes were prepared from LSD-treated animals. The translational inhibitor did not affect the spectrum of translation products from either set of polysomes.Abbreviations used HRI
heme-regulated inhibitor
- K
1000 Molecular weight
- LSD
d-lysergic acid diethylamide
- PMS
postmitochondrial supernatant
- TCA
trichloroacetic acid |
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Keywords: | Translational inhibitor LSD heat shock protein |
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