家蚕的基因文库

本文报导了家蚕Bombyx ,pro基因文库的构建。Sau3A 部分酶解的12-20kb家蚕染色体DNA片段被克隆在λEMBL4的BamHl位点，得到重组噬菌体数5.5×10⁵Pfu，超过了建库要求的理论值。进一步鉴定表明：重组噬菌体呈Spi^-表型；插入的DNA.片段各不相同；并巳从库内筛选到含有与蚊虫酮酶趴基因同源顺序的阳性重组体，这些结果显示了基因文库的可靠性。

A GENOMIC LIBRARY OF SILKWORM BOMBYXMORI

A genomic library of silkworm Bombyx rnori (Chinese species Dongfei×Huahe) was constructed. 12-20 kb DNA fragments from partially digested silkworm genomic DNA withsau3A were recovered and inserted into the BamHI site of the bacteriophage vector Λembl4. The recombinant molecules were packaged in vitro and amplified to a permanent library. The number of the bacteriophage recombinants was 5.5X10⁵ pfu. This number exceeded the theoretical number necessary to cover the whole genome. Futher examination showed: 1. The phenotype of bacteriophages in the library was Spi^-; 2. Different recombinants contained dif-ferent foreign DNA fragments; 3. After screening the library by hybridization, some recom binants containing the foreign DNA homologous to the probe of mosquito esterase BI gene had been isolated. These results demonstrated the reliability of the library.