Production of tylosin in solid-state fermentation by Streptomyces fradiae NRRL-2702 and its gamma-irradiated mutant (γ-1) |
| |
Authors: | Shazia Khaliq Nosheen Rashid Kalsoom Akhtar Muhammad Afzal Ghauri |
| |
Institution: | Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan |
| |
Abstract: | Aims: To develop solid-state fermentation system (SSF) for hyper production of tylosin from a mutant γ-1 of Streptomyces fradiae NRRL-2702 and its parent strain. Methods and Results: Various agro-industrial wastes were screened to study their effect on tylosin production in SSF. Wheat bran as solid substrate gave the highest production of 2500 μg of tylosin g?1 substrate by mutant γ-1 against parent strain (300 μg tylosin g?1 substrate). The tylosin yield was further improved to 4500 μg g?1 substrate 70% moisture, 10% inoculum (v/w), pH 9·2, 30°C, supplemental lactose and sodium glutamate on day 9]. Wild-type strain displayed less production of tylosin (655 μg of tylosin g?1 substrate) in SSF even after optimization of process parameters. Conclusion: The study has shown that solid-state fermentation system significantly enhanced the tylosin yield by mutant γ-1. Significance and Impact of the Study: This study proved to be very useful and resulted in 6·87 ± 0·30-fold increase in tylosin yield by this mutant when compared to that of wild-type strain. |
| |
Keywords: | gamma irradiation mutagenesis solid-state fermentation Streptomyces fradiae tylosin |
|
|